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Authors: Alison D. Pawlus , Emma Cantos-Villar , Tristan Richard , Jonathan Bisson ORCID , Pascal Poupard , Yorgos Papastamoulis , Jean-Pierre Monti , Pierre-Louis Teissedre , Pierre Waffo-Téguo , Jean-Michel Mérillon
Journal: Journal of Chromatography A (RoMEO status: Green)
Subjects:
Wine is a major dietary source of numerous potentially health promoting stilbenoids that have been the subject of many qualitative and quantitative studies. However, our initial HPLC–MS analyses of crude wine samples demonstrated the presence of compounds with molecular weights matching characteristic stilbenoid dimers, trimers, and tetramers that were unaccounted for in the literature. Due to the likelihood that these are known compounds, a chemical dereplication method is highly desirable. We developed such a method using LC-DAD–MS monitored fractionation steps, using adsorption and centrifugal partition chromatography (CPC), to obtain fractions rich in stilbenoids for analysis in stopped-flow LC–NMR.
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categories publications science
Authors: Alison D. Pawlus , Ramla Sahli , Jonathan Bisson ORCID , Céline Rivière , Jean-Claude Delaunay , Tristan Richard , Eric Gomès , Louis Bordenave , Pierre Waffo-Téguo , Jean-Michel Mérillon
Journal: Journal of Agricultural and Food Chemistry (RoMEO status: White)
Subjects:
NMR
We present stilbenoid profiles of canes from 16 grapevines. Fifteen stilbenoids were obtained through isolation and structure identification using MS, NMR, and [α]D or as commercial standards. An HPLC–UV method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of Vitis amurensis, Vitis arizonica, Vitis berlandieri, Vitis betulifolia, Vitis cinerea, Vitis × champini, Vitis × doaniana, Vitis labrusca, Vitis candicans (syn. Vitis mustangensis), Vitis riparia, Vitis rupestris, Vitis vinifera, Muscadinia rotundifolia, and a V.
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categories publications science
Authors: Jonathan Bisson ORCID , Pascal Poupard , Alison D. Pawlus , Alexandre Pons , Philippe Darriet , Jean-Michel Mérillon , Pierre Waffo-Téguo
Journal: Journal of Chromatography A (RoMEO status: Green)
Subjects:
CPC
MS
The phytochemical study of the root extract of the stilbenoid-rich Vitis riparia × Vitis berlandieri grapevine was carried out by centrifugal partition chromatography (CPC). For this reason, we developed a new elution mode we named back-step, which allowed us to obtain cleaner fractions and a more efficient separation process when used in conjunction with a classical elution approach. Three hydroxystilbenes: (E)-resveratrol, (E)-ɛ-viniferin and (E)-vitisin C, with greater than 90% purity were thus obtained through such process, with minimal sample handling and purification steps.
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categories publications science