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Authors: Yorgos Papastamoulis, Jonathan BissonORCID , Hamza Temsamani, Tristan Richard, Axel Marchal, Jean-Michel Mérillon, Pierre Waffo-Téguo
Journal: Tetrahedron (RoMEO status: Green)
Subjects: Pharmacognosy Phytochemistry Vine Wine Stilbenoids Polyphenols CPC Mass Spectrometry
Stilbenoids have received increasing attention over the last two decades since the discovery of resveratrol in wine. With an ever-growing rhythm, a multitude of biological activities of naturally occurring stilbenes are being reported. In this work, we investigated minor stilbenoid compounds from Vitis vinifera stalks. The compounds were purified by means of centrifugal partition chromatography (CPC), a countercurrent-separation technique. Electrospray ionization–ion trap mass spectrometry (ESI–IT-MS) after optimization proved to be extremely efficient for the detection of these new molecules, providing both structural information for structure elucidation and a means to achieve identification even with minute amounts.
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Authors: Merian Nassra, Stéphanie Krisa, Yorgos Papastamoulis, Gilbert Deccaux Kapche, Jonathan BissonORCID , Caroline André, Jan-Pieter Konsman, Jean-Marie Schmitter, Jean-Michel Mérillon, Pierre Waffo-Téguo
Journal: Planta Medica (RoMEO status: Blue)
Subjects: Pharmacognosy Phytochemistry Vine Wine Stilbenoids Polyphenols Bioactivity
Microglia-driven inflammatory processes are thought to play an important role in ageing and several neurological disorders. Since consumption of a diet rich in polyphenols has been associated with anti-inflammatory and neuroprotective effects, we studied the effects of twenty-five stilbenoids isolated from Milicia excelsa, Morus alba, Gnetum africanum, and Vitis vinifera. These compounds were tested at 5 and 10 µM on BV-2 microglial cells stimulated with bacterial lipopolysaccharide. Ten stilbenoids reduced lipopolysaccharide-induced nitric oxide production at 5 and/or 10 µM.
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Authors: Alison D. Pawlus, Emma Cantos-Villar, Tristan Richard, Jonathan BissonORCID , Pascal Poupard, Yorgos Papastamoulis, Jean-Pierre Monti, Pierre-Louis Teissedre, Pierre Waffo-Téguo, Jean-Michel Mérillon
Journal: Journal of Chromatography A (RoMEO status: Green)
Subjects: Analytical Chemistry Dereplication LC-NMR Vine Wine
Wine is a major dietary source of numerous potentially health promoting stilbenoids that have been the subject of many qualitative and quantitative studies. However, our initial HPLC–MS analyses of crude wine samples demonstrated the presence of compounds with molecular weights matching characteristic stilbenoid dimers, trimers, and tetramers that were unaccounted for in the literature. Due to the likelihood that these are known compounds, a chemical dereplication method is highly desirable. We developed such a method using LC-DAD–MS monitored fractionation steps, using adsorption and centrifugal partition chromatography (CPC), to obtain fractions rich in stilbenoids for analysis in stopped-flow LC–NMR.
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Authors: Carole Lambert, Jonathan BissonORCID , Pierre Waffo-Téguo, Yorgos Papastamoulis, Tristan Richard, Marie-France Corio-Costet, Jean-Michel Mérillon, Stéphanie Cluzet
Journal: Journal of Agricultural and Food Chemistry (RoMEO status: White)
Subjects: Pharmacognosy Phytochemistry Vine Wine Stilbenoids Polyphenols Bioactivity Antifungal
The interaction between Vitis vinifera and trunk disease fungi requires better understanding. We studied the role of phenolics as possible plant defense compounds in this context. The impact of 24 grapevine phenolic compounds was determined on 6 major wood decay fungi by an in vitro agar plate assay. Hydroxystilbenoids, especially oligomers such as miyabenol C, isohopeaphenol, and vitisin A and B, greatly reduced the growth of the fungi, except that of Phaeoacremonium aleophilum.
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